fig8

Figure 8. Clac10 (5c) and Clac12 (5e) suppress the growth of HCT116-tumor xenografts in mice. (A) Immunofluorescence reveals that compounds 5c (Clac10) and 5e (Clac12) reduce the number of proliferating cells (Ki67-positive nuclei) and increase the expression of cleaved caspase-3 in HCT116-xenograft tumors in mice; Quantification of Ki-67-positive cells (B) and cleaved caspase-3 staining (C) was performed; (D) Western blot analyses of tissue lysates from animals treated with 5c (Clac10) and 5e (Clac12) show significantly higher levels of cleaved PARP, indicating apoptosis, and reduced p-GSK3B expression, indicating inhibition of histamine-signaling. Data is presented as mean ± SEM. A statistical comparison was performed using an ordinary one-way ANOVA. PARP: Poly (ADP-ribose) polymerase; p-GSK3B: phosphorylated-glycogen synthase kinase 3 beta; SEM: standard error of the mean; ANOVA: analysis of variance.