fig7

Figure 7. Clac10 (5c) and Clac12 (5e) reduce the growth of colon cancer xenografts in NSG mice. (A) HCT116 cells were injected into both flanks of NSG mice (n = 5), and tumors (n = 10; each mouse carrying 2 xenograft tumors, totaling 10 tumors per group) were allowed to develop until palpable. Then, Clac10 and Clac12 (20 mg/kg body weight) were injected intraperitoneally daily for 24 days; At the end of the study, the tumors were removed, imaged (B), and analyzed further; (C) The tumors in Clac10-treated mice were smaller than those in the control group; (D) Treatment with Clac10 and Clac12 resulted in significantly lower tumor volumes than in the control group. Tumor volume was measured weekly. The data are shown as mean ± SEM. We used ordinary one-way ANOVA for statistical comparisons. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001. SEM: Standard error of the mean; ANOVA: analysis of variance.