fig4

From: Novel N-chloroacetyl-2-pyrazoline analogs with 2-naphthyl and ferrocenyl groups: targeting histamine receptor H1 to overcome colorectal cancer drug resistance

Novel <i>N</i>-chloroacetyl-2-pyrazoline analogs with 2-naphthyl and ferrocenyl groups: targeting histamine receptor H1 to overcome colorectal cancer drug resistance

Figure 4. Clac10 (5c) and Clac12 (5e) suppress spheroid formation. (A) HCT116 and DLD1 cells were cultured in specialized spheroid media in ultra-low attachment plates and treated with IC₅₀ and ½IC₅₀ concentrations of Clac10 (5c) and Clac12 (5e). The spheroids were imaged at 10X magnification. After 5 days, colonospheres were imaged at 10X magnification; (B) Spheroid count from the spheroid formation assay. Clac10 (5c) and Clac12 (5e) treatments significantly reduced the number of colonospheres. Data is shown as mean ± SD. An ordinary one-way ANOVA was used for statistical analysis. IC₅₀: Half maximal inhibitory concentration; SD: standard deviation; ANOVA: analysis of variance.