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Figure 3. Clac10 (5c) and Clac12 (5e) trigger apoptosis. (A) HCT116 and DLD1 cells were treated with the IC₅₀ concentration of Clac10 (5c) and Clac12 (5e) for 48 h, stained with Annexin V (FITC) and PI, and analyzed by flow cytometry; (B) Quantification shows that both compounds significantly induced late apoptosis in HCT116 and DLD1 cells; (C) Lysates from HCT116 and DLD1 cells treated with Clac10 (5c) and Clac12 (5e) showed reduced levels of anti-apoptotic marker proteins BCL-XL, MCL-1, and BCL-2 compared to untreated controls. Data is presented as mean ± SD. An ordinary two-way ANOVA was used for statistical comparison. IC₅₀: Half maximal inhibitory concentration; FITC: fluorescein isothiocyanate; PI: propidium iodide; BCL-XL: B-cell lymphoma-extra large; MCL-1: myeloid cell leukemia 1; BCL-2: B-cell lymphoma 2; SD: standard deviation; ANOVA: analysis of variance; BAX: BCL2-associated X protein.