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Figure 2. Clac10 (5c) and Clac12 (5e) cause cell cycle arrest in CRC cells. (A) HCT116 and (B) DLD1 cells were treated with IC₅₀ and ½IC₅₀ concentrations of 5c (Clac10) and 5e (Clac12) for 48 h and analyzed by flow cytometry after propidium iodide staining for DNA content; (C) The quantification figure shows that both compound treatments led to cell cycle arrest, as evidenced by an increase in the Sub-G0 cell population; (D) Lysates from HCT116 and DLD1 cells treated with IC₅₀ concentrations of 5c (Clac10) and 5e (Clac12) for 48 h were examined by western blotting. Both compounds significantly reduced cyclin D1 expression. Data is presented as mean ± SD. An ordinary two-way ANOVA was used for statistical comparison. CRC: Colorectal cancer; IC₅₀: half maximal inhibitory concentration; SD: standard deviation; ANOVA: analysis of variance; PI: propodium iodide.