fig1
Figure 1. Clac10 (5c) and Clac12 (5e) inhibit proliferation and colony formation in CRC cell lines HCT116 and DLD1. (A) Cells treated with 0-10 μM of each compound for up to 72 h showed a dose- and time-dependent reduction in proliferation, indicating antiproliferative effects; (B) Cells treated with various concentrations of 5c (Clac10) and 5e (Clac12) for 48 h, followed by 10-14 days of culture in compound-free media, revealed that both compounds significantly reduced colony formation, indicating suppressed clonogenic potential; (C) Quantification confirmed a significant decrease in colony numbers in treated cells compared with vehicle-treated cells. The data were expressed as mean ± SD and analyzed by one-way ANOVA. CRC: Colorectal cancer; SD: standard deviation; ANOVA: analysis of variance; IC50: half maximal inhibitory concentration.
