fig3

From: Stimuli-responsive nanomaterials as modulators of cancer stem cell fate: current progress and future perspectives

Figure 3. (A) Schematic synthetic route of DFHC and mechanisms of DFHC-regulated TAMs inducing ferroptosis of CSCs in the nebulized therapy of early orthotopic lung cancer; (B) Immunostaining results of CSCs (dark turquoise), TAMs (green), DFHC (red), and nucleus (blue, DAPI) in lung regions. Scale bar: 25 μm. Reproduced with permission^[61]. Copyright 2023, American Chemical Society; (C) Schematic illustration of the ferroptosis amplification strategy to prevent tumor recurrence and metastasis after resection surgery; (D) Western blot analysis of the expression of SLC7A11 and GPX4 in CSCs under different treatments; (E) qPCR analysis of the expression of stemness-related genes in CSCs after treatment. Reproduced with permission^[63]. Copyright 2024, American Chemical Society. HMSN: Hollow mesoporous silica nanoparticles; DFHC: TAM: tumor-associated macrophage; CSC: cancer stem cell; FPN: DMT: PUFA: PL: ALOX: GPX4: glutathione peroxidase 4; GSH: NADPH: GFP: FC: SLC7A11: DKK1: MMP7: NANOG: OCT4: octamer-binding transcription factor 4; SOX2: SRY-Box transcription factor 2; LLC: DAPI: qPCR: