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Figure 5. Effects of zinc gluconate on migrasomes and platelet dynamics in mice. (A) Zinc gluconate treatment inhibited migrasome formation in mouse blood. Scale bar: 40 µm; (B) Statistical analysis based on (A) (n = 11 mice per group); (C) Platelet counts in mouse blood before and 24 h after zinc gluconate treatment were statistically evaluated (n = 6 mice per group); (D) Images representative of aggregated platelets in blood smears from zinc gluconate-treated mice. Scale bar: 40 µm; (E) Evaluation of peripheral vascular bleeding after 24 h of zinc gluconate treatment in mice (n = 6 mice per group); (F) Statistical analysis of the data in (E); (G) Peripheral vascular bleeding in mice after 24 h of zinc gluconate treatment vs. untreated controls (n = 6 mice per group). Values are mean ± SEM. ^**P < 0.01; ^****P < 0.0001; (H) Diagram illustrating the effect of reduced migrasome levels on blood clotting. SEM: Standard error of the mean.