fig4

Figure 4. Effects of ZnSO₄ on migrasomes and platelet dynamics in mice. (A) Schematic overview of the migrasome isolation workflow from mouse blood. In step 1, crude migrasomes are collected by concentration. In step 2, migrasomes are further purified with the Migralso^TM Pro Mouse Immune Cell Extraction Kit; (B) ZnSO₄ treatment inhibited migrasome formation in mouse blood. Scale bar: 40 µm; (C) Statistical evaluation of the data in (B) (n = 11 mice per group); (D) Platelet counts in mouse blood at 0 and 24 h after ZnSO₄ treatment were statistically analyzed (n = 6 mice per group); (E) Representative images of platelet aggregates in blood smears from ZnSO₄-treated mice. Scale bar: 40 µm; (F) Assessment of peripheral vascular bleeding in mice after 24 h of ZnSO₄ treatment (n = 6 mice per group); (G) Statistical analysis of the results in (F); (H) Peripheral vascular bleeding was compared between mice incubated with ZnSO₄ for 24 h and untreated controls (n = 6 mice per group). Data are shown as mean ± SEM. ^**P < 0.01; ^****P < 0.0001; (I) Schematic showing how blood migrasomes affect platelet function and coagulation dynamics. SEM: Standard error of the mean; WGA: wheat germ agglutinin.