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Figure 1. EGFR-TKI resistance induces M2 polarization and inhibits M1 polarization of TAMs in NSCLC. (A and B) qRT-PCR analysis of the expression M1/M2-like phenotypic biomarkers of THP-1 cells cultured with CM from EGFR-TKI-sensitive or -resistant PC9 and HCC827 cells. Data represent results from three independent experiments; (C) Flow cytometry analysis of the percentages of M1/M2-like phenotype macrophages in THP-1 cells intervened by CM from EGFR-TKI-sensitive or -resistant PC9 and HCC827 cells. Bar graphs show average values from three independent experiments; (D and E) The percentages of CD86⁺ and CD206⁺ TAMs in F4/80⁺ TAMs in tumor tissues from EGFR-TKI-sensitive or -resistant PC9 and HCC827 CDX mouse models were analyzed by flow cytometry; (D) Representative pseudo-color plots presenting the process of gating; (E) Average results from three independent experiments. ^*P < 0.05, ^**P < 0.005, ^***P < 0.0005, ^****P < 0.0001.