fig7

Figure 7. Effects of neuronal TMPRSS6 knockdown on the BMP/Smad signaling pathway. (A and B) Western blot images of HJV in the hippocampus, and quantification; (C and D) Western blot images of p-Smad1/5 in the hippocampus, and quantification; (E and F) Western blot images of Smad1 in the hippocampus, and quantification; (G and H) Western blot images of Smad4 in the hippocampus, and quantification; (I) qRT-PCR evaluation of Hepcidin mRNA levels in the hippocampus; (J and K) Western blot images of FPN1 in the hippocampus, and quantification. The data are presented as the mean ± SEM. ^*P < 0.05, ^**P < 0.01. BMP: Bone morphogenic protein; HJV: Hemojuvelin; qRT-PCR: quantitative reverse transcription polymerase chain reaction; FPN1: ferroportin 1; SEM: standard error of the mean.