fig3

Figure 3. Validation of neuronal knockdown of TMPRSS6. (A) qRT-PCR analysis of TMPRSS6 mRNA in the hippocampus; (B and C) Western blot images of TMPRSS6 in the hippocampus and its quantification; (D-G) Immunofluorescence of TMPRSS6 protein in the CA3 (D) and DG (F) regions and its quantification. (scale bar: 50 μm). NeuN: Label neurons; DAPI: Label nucleus. The data are presented as the mean ± SEM. ^*P< 0.05, ^**P < 0.01. qRT-PCR: Quantitative reverse transcription polymerase chain reaction; SEM: standard error of the mean.