fig6

Figure 6. mRNA expression of proinflammatory and ferroptotic markers in fetal (D135) hearts from ewes receiving either control or overnutrition (150% NRC recommendation) for 135 days assessed using quantitative real-time PCR (qPCR). (A) CD14 mRNA; (B) CD68 mRNA; (C) IL1A mRNA; (D) IL1B mRNA; (E) IL6 mRNA; (F) IL18 mRNA; (G) TLR4 mRNA; (H) iNOS mRNA; (I) M-CSF mRNA; (J) GPX4 mRNA; and (K) SLC7A11 mRNA. All gene expressions were normalized to that of 18S (as house-keeping gene). Mean ± SEM, *P < 0.05 between groups, n = 6 fetuses per group.