fig4

Figure 4. Immunoblot analysis of markers of bacterial sEVs in BME preparations and lysates of E. coli. and L gasseri. (A) groEL; (B) LPS; and (C) LTA. Dashed boxes identify the expected position of markers that were not detected in BME preparations; solid box identify markers in bacterial lysates (control). M, marker. sEV: Small extracellular vesicle; BME: bovine milk small extracellular vesicle; LPS: lipopolysaccharide; LTA: lipoteichoic acid.