fig9

Figure 9. Activation of ER stress shows a positive association with the upregulation of ACACA in HCC. (A) Western blotting analysis of the biomarkers of ER stress in HCC tissues and paired non-tumor tissues; (B) Typical immunohistochemistry images of GRP78 protein in HCC tissues and paired liver tissues (scale bars: 100 μm for upper panels, 20 μm for lower panels); (C) Western blotting examination of ER stress markers after co-incubation with different concentrations of FFA for 24 h; (D) Western blotting analysis of the expression of ER stress markers and ACACA in HepG2 cells after co-incubation with different concentrations of TM for 24 h; (E) Heatmap of the correlation of the ER stress marker genes and ACACA according to TCGA-LIHC databases. ER: Endoplasmic reticulum; HCC: Hepatocellular carcinoma; FFA: free fatty acids; TM: tunicamycin; TCGA: The Cancer Genome Atlas; LIHC: Liver Hepatocellular Carcinoma. ^*P < 0.05.