fig8

Figure 8. Overexpression of ACACA is correlated with poor overall survival and adverse pathological characteristics in HCC patients. (A) ACACA expression levels in 33 kinds of tumor tissues and their matched non-tumor tissues, analyzed using the TCGA database. (B) ACACA mRNA profiles in HCC tissues compared to adjacent non-tumor tissues, based on TCGA data. ACACA expression across different pathologic T stages (C and D) and histological grade groups (E), according to TCGA. (F) Representative IHC staining images of ACACA in HCC and matched non-tumor tissues, with (G) quantification of IHC staining scores (scale bars: 100 μm for upper panels, 20 μm for lower panels). Western blotting analysis of ACACA expression in freshly resected HCC tissues (H) and HCC cell lines (I). (J) Kaplan-Meier analysis comparing OS between patients with high ACACA expression (n = 27) and low ACACA expression (n = 23). HCC: Hepatocellular carcinoma; TCGA: The Cancer Genome Atlas; IHC: immunohistochemistry; OS: overall survival. ^*P < 0.05; ^**P < 0.01; ^***P < 0.001.