fig10

From: Screening for lipid metabolism-related genes and identifying the therapeutic potential of ACACA for ER stress-related progression in hepatocellular carcinoma

Screening for lipid metabolism-related genes and identifying the therapeutic potential of ACACA for ER stress-related progression in hepatocellular carcinoma

Figure 10. ACACA promotes proliferation in HCC. Utilization of Western blotting to analyze ACACA expression in HepG2 cells treated with TM (A) or a combination of PA and fenofibrate (B). qRT-PCR assays measured the expression of ACACA (C), FASN (D), PPAR-γ (E), and C/EBP-β (F) following treatment with PA and fenofibrate. (G) Western blotting analysis of the effects of fenofibrate or 4-PBA on ACACA expression levels in TM-treated HepG2 cells. (H) Cell viability was assessed using the CCK-8 assay. HCC: Hepatocellular carcinoma; TM: tunicamycin; PA: palmitic acid; qRT-PCR: quantitative real-time polymerase chain reaction; 4-PBA: 4-phenylbutyric acid; CCK-8: Cell counting kit-8. ^*P < 0.05; ^**P < 0.01; ^***P < 0.001.